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KMID : 0604520150410040375
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Journal of the Society of Cosmetic Scientists of Korea
2015 Volume.41 No. 4 p.375 ~ p.382
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Isolation of Stenotrophomonas rhizopilae Strain GFC09 with Ginsenoside Converting Activity and Anti-wrinkle Effects of Converted Ginsenosides
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Min Jin-Woo
Kim Hye-Jin
Joo Kwang-Sik
Kang Hee-Cheol
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Abstract
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Ginsenosides (ginseng saponin) as the one of important pharmaceutical compounds of ginseng and is responsible for the pharmacological and biological activities. These ginsenoside produces diverse small molecules ginsenoside which have more pharmacological activities including anti-wrinkle, anti-cancer and anti-oxidant effects. In the present study, we isolated bacteria using esculin agar, to produce β-glucosidase, and we focused on the bio-transformation of ginsenoside. Phylogenetic tree analysis was performed by comparing the 16S rRNA sequences; we identified the strain as Stenotrophomonas rhizopilae strain GFC09. In order to determine the optimal conditions for enzyme activity, the crude enzyme was incubated with 1 mM ginsenoside Rb1. Bioconversion of ginsenoside Rb1 were analyzed using TLC and HPLC. The crude enzyme hydrolyzed the ginsenoside Rb1 along the following pathway: LB: Rb1→Rd→F2 into compound K, TSB: Rb1→Rd→F2. The structure of the hydrolyzed metabolites were identified by NMR. The activity screening tests showed that the conversion product induced the production of type I procollagen in a dose-dependent manner. These results suggested that hydrolyzed ginseng product containing the ginsenoside F2 and compound K could be useful as an active ingredient for wrinkle-care cosmetics.
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KEYWORD
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Stenotrophomonas rhizopilae strain GFC09, ginsenoside compound K, anti-wrinkle, bioconversion
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